High throughput topology determination

Although theoretical protein topology prediction methods have high accuracy, they can predict the topology correctly for only 70-80% of the proteome. Unfortunately molecular biology experimental techniques applied in the verification of the predicted transmembrane protein topology (e. g. epitope insertion, examination of glycosylation state) are very slow and expensive. Furthermore, if modification happens at an inadequate site (functional sites, membrane-embedded regions, etc.) experimental results may prove difficult to interpret or may even be misleading. However, incorporating the results of well performed experiments as constraints into the hidden Markov model based topology prediction methods will make our prediction notably more accurate. This prediction then may be utilized during the design of further experiments.

People working on this project

Gábor E. Tusnády

Collaborating partners

Gergely Szakács Institute of Enzymology, RCNS, HUN-REN

Beáta G Vértessy Institute of Enzymology, RCNS, HUN-REN

Katalin Folk Medzihradszky Laboratorium of Proteomics Research, BRC, HUN-REN

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